ctla4 b7 1 (BPS Bioscience)
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ctla4 b7 1
Ctla4 B7 1, supplied by BPS Bioscience, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ctla4+b7+1/CTLA4%3AB7-1%5BBiotinylated%5D+Inhibitor+Screening+Assay+Kit/us12565517-371-9-14
Average 92 stars, based on 7 article reviews
Ctla4 B7 1, supplied by BPS Bioscience, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ctla4+b7+1/CTLA4%3AB7-1%5BBiotinylated%5D+Inhibitor+Screening+Assay+Kit/us12565517-371-9-14
Average 92 stars, based on 7 article reviews
ctla4 b7 1 - by Bioz Stars,
2026-09
92/100 stars
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Inhibition:Article Title: Cyclic peptide having CTLA-4 inhibitory activity and use thereof Article Snippet: IC50 can be determined by using, for example, competitive ELISA, surface plasmon resonance (BIAcore (trademark)) analysis or the like. .. IC50 value for the cyclic peptide of the present invention with respect to its inhibition of binding between human CTLA-4 and human CD80 is preferably measured by Binding Assay:Article Title: Cyclic peptide having CTLA-4 inhibitory activity and use thereof Article Snippet: IC50 can be determined by using, for example, competitive ELISA, surface plasmon resonance (BIAcore (trademark)) analysis or the like. .. IC50 value for the cyclic peptide of the present invention with respect to its inhibition of binding between human CTLA-4 and human CD80 is preferably measured by Article Title: Cyclic peptide having CTLA-4 inhibitory activity and use thereof Article Snippet: .. Inhibitory activity was measured using the Screening Assay:Article Title: Cyclic peptide having CTLA-4 inhibitory activity and use thereof Article Snippet: IC50 can be determined by using, for example, competitive ELISA, surface plasmon resonance (BIAcore (trademark)) analysis or the like. .. IC50 value for the cyclic peptide of the present invention with respect to its inhibition of binding between human CTLA-4 and human CD80 is preferably measured by Article Title: Cyclic peptide having CTLA-4 inhibitory activity and use thereof Article Snippet: .. Inhibitory activity was measured using the Article Title: Cyclic peptide having CTLA-4 inhibitory activity and use thereof Article Snippet: .. Sample name −2 −1 0 1 2 3 4 5 6 7 8 9 10 11 12 0 +1 +2 (μM) ID NO: Original C4-302mat-2-1-5 G G C M H P F L L V V S H H F C G G 3.921 29 #19 C4-302mat-3-1-27 G G C M H P F L P I V S H H F C E R 0.075 48 #10 C4-302mat-3-100-44 G G C L H P F L P I V S H H F C G R 0.082 39 #25 C4-302mat-3-1-12 G G C M H P F L P V V S R H F C G R 0.085 53 #20 C4-302mat-3-1-23 G G C M H P F L P I A S L H F C G R 0.160 49 #3 C4-302mat-3-1-48 G G C M H P F L P V V S H H F C G A 0.187 32 #18 C4-302mat-2-100-27 G G C M H P F L P I V S H H F C G S 0.189 47 #16 C4-302mat-3-100-12 G G C M H P Y L P V I S H H F C G R 0.217 45 #6 C4-302mat-3-1-22 G G C M H P F L P V A S H H F C G R 0.222 35 #4 C4-302mat-3-100-37 G G C M H P F L P V V S H H F C G R 0.225 33 #5 C4-302mat-1-1-35 G G C M H P F L P V A S H H F C G G 0.288 34 #11 C4-302mat-3-1-45 G G C R H P F L P I V S R H F C R G 0.300 40 #21 C4-302mat-3-1-32 G G C M H P F L P I L S H H F C A G 0.322 50 #23 C4-302mat-3-1-31 G S C M H P F L P V A S R H F C G R 0.342 51 #7 C4-302mat-3-100-27 G G C M H P F L P V A S H H F C A G 0.352 36 #2 C4-302mat-2-100-18 G G C M H P F L P V V S H H F C R G 0.369 31 #8 C4-302mat-2-1-2 G G C L H P F L P V V S H H F C G D 0.474 37 #22 C4-302mat-3-1-35 G G C M H P F L Q T V S H H F C G G 0.668 54 #24 C4-302mat-3-1-5 G G C M H P F L P V V S R H F C G G 0.674 52 #14 C4-302mat-3-1-10 G G C K H P Y L P V A S H H F C R G 0.703 43 #17 C4-302mat-2-1-3 G G C M H P F L P V T S H H F C G G 0.826 46 #13 C4-302mat-3-100-9 G G C K H P F L P V V S H H F C G G 0.832 42 #9 C4-302mat-2-1-38 G G C L H P F L P L V S H H F C G G 0.867 38 #12 C4-302mat-3-100-20 G G C V H P F L P V V S H H F C R G 0.889 41 #1 C4-302mat-1-1-24 G G C M H P F L P V V S H H F C G G 1.035 30 #32 C4-302mat-1-1-37 G G C M H P F L R V V S H H F C G G 1.493 61 #15 C4-302mat-2-1-27 G G C M H P Y L P V I S H H F C G G 1.637 44 #27 C4-302mat-2-100-4 G G C M H P F L Q V V S H H F C G G 4.181 56 (6) Evaluation of Inhibitory Activity after Affinity Maturation (IC50 Measurement) For clone #19 which showed high affinity in the EC50 measurement, IC50 of the MBP fusion protein was measured using the Article Title: Cyclic peptide having CTLA-4 inhibitory activity and use thereof Article Snippet: Inhibitory activity was measured using the CTLA4:B7-1 [Biotinylated] Inhibitor Screening Assay Kit (BPS Biosciences) as described above. (18) Identification of Amino Acids Essential for CTLA-4 Binding in High Affinity Clones by Alanine Scanning Using the CTLA-4 binding clone #10 (CLHPFLPIVSHHFCGR) (SEQ ID NO: 27), which had high affinity, as the original clone, a series of oligo DNAs encoding mutants in which any one amino acid residue except cysteine was sequentially replaced with alanine were synthesized and each oligo DNA was subcloned into pET-Mal vector (FIG. 2), and the binding activity of the alanine substitution mutants to CTLA-4 was evaluated by ELISA as described above. (19) Affinity Maturation with Random Library Oligo DNAs of a library were synthesized (see below), in which amino acid residues (positions 1, 4, 8, 10, +1 and +2) (corresponding to X1, X1, X8, X10, X+1 and X+2) other than those essential or advantageous for CTLA-4 binding (2H, 3P, 5L, 6P, 7I, 9S, 11H, 12F) identified by alanine scanning were randomized (NNS). (No. 19) NNS6: (SEQ ID NO: 28) GACTATAAAGATGACGATGACAAAGGCGGTTGCNNSCATCCANNSCTGC CGATANNSTCANNSCATTTCTGTNNSNNSGGATCCGAGCAGAAGCTGAT CTCTGAGGAGGATCTG The gene format for ribosome display (coupling with 5′ UTR and g3p) was prepared in the same manner as described above. .. The measurement of inhibitory activity was performed using the Activity Assay:Article Title: Cyclic peptide having CTLA-4 inhibitory activity and use thereof Article Snippet: .. Inhibitory activity was measured using the Article Title: Cyclic peptide having CTLA-4 inhibitory activity and use thereof Article Snippet: .. Sample name −2 −1 0 1 2 3 4 5 6 7 8 9 10 11 12 0 +1 +2 (μM) ID NO: Original C4-302mat-2-1-5 G G C M H P F L L V V S H H F C G G 3.921 29 #19 C4-302mat-3-1-27 G G C M H P F L P I V S H H F C E R 0.075 48 #10 C4-302mat-3-100-44 G G C L H P F L P I V S H H F C G R 0.082 39 #25 C4-302mat-3-1-12 G G C M H P F L P V V S R H F C G R 0.085 53 #20 C4-302mat-3-1-23 G G C M H P F L P I A S L H F C G R 0.160 49 #3 C4-302mat-3-1-48 G G C M H P F L P V V S H H F C G A 0.187 32 #18 C4-302mat-2-100-27 G G C M H P F L P I V S H H F C G S 0.189 47 #16 C4-302mat-3-100-12 G G C M H P Y L P V I S H H F C G R 0.217 45 #6 C4-302mat-3-1-22 G G C M H P F L P V A S H H F C G R 0.222 35 #4 C4-302mat-3-100-37 G G C M H P F L P V V S H H F C G R 0.225 33 #5 C4-302mat-1-1-35 G G C M H P F L P V A S H H F C G G 0.288 34 #11 C4-302mat-3-1-45 G G C R H P F L P I V S R H F C R G 0.300 40 #21 C4-302mat-3-1-32 G G C M H P F L P I L S H H F C A G 0.322 50 #23 C4-302mat-3-1-31 G S C M H P F L P V A S R H F C G R 0.342 51 #7 C4-302mat-3-100-27 G G C M H P F L P V A S H H F C A G 0.352 36 #2 C4-302mat-2-100-18 G G C M H P F L P V V S H H F C R G 0.369 31 #8 C4-302mat-2-1-2 G G C L H P F L P V V S H H F C G D 0.474 37 #22 C4-302mat-3-1-35 G G C M H P F L Q T V S H H F C G G 0.668 54 #24 C4-302mat-3-1-5 G G C M H P F L P V V S R H F C G G 0.674 52 #14 C4-302mat-3-1-10 G G C K H P Y L P V A S H H F C R G 0.703 43 #17 C4-302mat-2-1-3 G G C M H P F L P V T S H H F C G G 0.826 46 #13 C4-302mat-3-100-9 G G C K H P F L P V V S H H F C G G 0.832 42 #9 C4-302mat-2-1-38 G G C L H P F L P L V S H H F C G G 0.867 38 #12 C4-302mat-3-100-20 G G C V H P F L P V V S H H F C R G 0.889 41 #1 C4-302mat-1-1-24 G G C M H P F L P V V S H H F C G G 1.035 30 #32 C4-302mat-1-1-37 G G C M H P F L R V V S H H F C G G 1.493 61 #15 C4-302mat-2-1-27 G G C M H P Y L P V I S H H F C G G 1.637 44 #27 C4-302mat-2-100-4 G G C M H P F L Q V V S H H F C G G 4.181 56 (6) Evaluation of Inhibitory Activity after Affinity Maturation (IC50 Measurement) For clone #19 which showed high affinity in the EC50 measurement, IC50 of the MBP fusion protein was measured using the Article Title: Cyclic peptide having CTLA-4 inhibitory activity and use thereof Article Snippet: Inhibitory activity was measured using the CTLA4:B7-1 [Biotinylated] Inhibitor Screening Assay Kit (BPS Biosciences) as described above. (18) Identification of Amino Acids Essential for CTLA-4 Binding in High Affinity Clones by Alanine Scanning Using the CTLA-4 binding clone #10 (CLHPFLPIVSHHFCGR) (SEQ ID NO: 27), which had high affinity, as the original clone, a series of oligo DNAs encoding mutants in which any one amino acid residue except cysteine was sequentially replaced with alanine were synthesized and each oligo DNA was subcloned into pET-Mal vector (FIG. 2), and the binding activity of the alanine substitution mutants to CTLA-4 was evaluated by ELISA as described above. (19) Affinity Maturation with Random Library Oligo DNAs of a library were synthesized (see below), in which amino acid residues (positions 1, 4, 8, 10, +1 and +2) (corresponding to X1, X1, X8, X10, X+1 and X+2) other than those essential or advantageous for CTLA-4 binding (2H, 3P, 5L, 6P, 7I, 9S, 11H, 12F) identified by alanine scanning were randomized (NNS). (No. 19) NNS6: (SEQ ID NO: 28) GACTATAAAGATGACGATGACAAAGGCGGTTGCNNSCATCCANNSCTGC CGATANNSTCANNSCATTTCTGTNNSNNSGGATCCGAGCAGAAGCTGAT CTCTGAGGAGGATCTG The gene format for ribosome display (coupling with 5′ UTR and g3p) was prepared in the same manner as described above. .. The measurement of inhibitory activity was performed using the Clone Assay:Article Title: Cyclic peptide having CTLA-4 inhibitory activity and use thereof Article Snippet: .. Inhibitory activity was measured using the Residue:Article Title: Cyclic peptide having CTLA-4 inhibitory activity and use thereof Article Snippet: .. Inhibitory activity was measured using the Synthesized:Article Title: Cyclic peptide having CTLA-4 inhibitory activity and use thereof Article Snippet: .. Inhibitory activity was measured using the Plasmid Preparation:Article Title: Cyclic peptide having CTLA-4 inhibitory activity and use thereof Article Snippet: .. Inhibitory activity was measured using the |